Disparate Tissue-specific Expression of Members of the Tissue Kallikrein Multigene Family of the Rat

• DOI: 10.1074/jbc.271.23.13684
• Publication Date: 2002-07-26T14:52:05Z
• Authors: Raymond J. MacDonald; E. Michelle South...; Evert Kroon
• Keywords: Male; 0301 basic medicine; Base Sequence; Genetic Linkage; Molecular Sequence Data; Submandibular Gland; Gene Expression; Polymerase Chain Reaction; Sensitivity and Specificity; Rats; 03 medical and health sciences; Multigene Family; Animals; Female; Kallikreins; Tissue Distribution; RNA, Messenger; DNA Primers

To understand the regulatory diversity of rat family linked kallikrein genes, we have assayed expression members in 20 major organs. Reverse transcription-polymerase chain reaction analysis using primers and hybridization probes specific for each 10 expressed genes showed that no two share same organ-specific pattern expression. The only common site all known active is submandibular gland. presence mRNA at least one member detected 19 these organs (liver excepted), from as few three to many 18 individual members. For there can be more than a 105-fold variation levels among organs, limit detection slightly less 1 molecule/10 cells 10,000 molecules/cell. Despite high sequence conservation close linkage, this are very different complex patterns. A gradient corresponds order within locus.