ORE, a Eukaryotic Minimal Essential Osmotic Response Element

• DOI: 10.1074/jbc.271.31.18318
• Publication Date: 2002-07-26T14:50:28Z
• Authors: Joan D. Ferraris; Chester K. Williams; Kyu-Yong Jung; Jennifer J. Bedford; Maurice B. Burg; Arlyn García-Pérez
• Keywords: 0301 basic medicine; Base Sequence; Molecular Sequence Data; NF-kappa B; DNA; Water-Electrolyte Balance; Transfection; Cell Line; 03 medical and health sciences; Dogs; Gene Expression Regulation; Aldehyde Reductase; Genes, Reporter; Osmotic Pressure; Consensus Sequence; Animals; Sorbitol; Rabbits; Cloning, Molecular; Luciferases

Organisms, almost universally, adapt to hyperosmotic stress through increased accumulation of organic osmolytes but the molecular mechanisms have only begun be addressed. Among mammalian tissues, renal medullary cells are uniquely exposed extreme stress. Sorbitol, synthesized aldose reductase, is a predominant osmolyte induced under conditions in cells. Using rabbit cell line, we originally demonstrated that induces transcription reductase gene. Recently, cloned gene, characterized its structure, and found first evidence an osmotic response region eukaryotic Now, progressively subdivided this 3221-base pair (bp) into discrete fragments reporter gene constructs. Thereby, functionally defined smallest sequence able confer on downstream independent other putative cis-elements, is, minimal essential element (ORE). The ORE CGGAAAATCAC(C) (bp -1105/-1094). A 17-bp fragment (-1108/-1092) containing used as probe electrophoretic mobility shift assays suggests induction slowly migrating band. Isolation trans-acting factor(s) characterization their interaction with should elucidate basic for regulation expression by