Regulation of Lung Cancer Cell Migration and Invasion by Reactive Oxygen Species and Caveolin-1

• DOI: 10.1074/jbc.m110.124958
• Publication Date: 2010-10-06T00:13:41Z
• Authors: Sudjit Luanpitpong; Siera Jo Talbott; Yon Rojanasakul; Ubonthip Nimmannit; Varisa Pongrakhan...; Liying Wang; Pithi Chanvorachote
• Keywords: 0303 health sciences; Lung Neoplasms; Skin Neoplasms; Cell Survival; Caveolin 1; Down-Regulation; Epithelial Cells; 3. Good health; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Humans; Neoplasm Invasiveness; RNA Interference; Neoplasm Metastasis; Reactive Oxygen Species; Melanoma

The acquired capability of tumor cells to migrate and invade neighboring tissues is associated with high metastatic potential and advanced stage of cancers. Recently, signaling molecules such as reactive oxygen species (ROS) and caveolin-1 (Cav-1) have been implicated in the aggressive behavior of cancer cells. However, the roles of specific ROS in cancer cell migration and Cav-1 regulation are unclear. We demonstrate here that Cav-1 plays an important role in the migration and invasion of human lung carcinoma H460 cells and that these effects are differentially regulated by cellular ROS. Using various known inhibitors and donors of ROS, we found that different ROS have different effects on Cav-1 expression and cell migration and invasion. Superoxide anion and hydrogen peroxide down-regulated Cav-1 expression and inhibited cell migration and invasion, whereas hydroxyl radical up-regulated the Cav-1 expression and promoted cell migration and invasion. The down-regulating effect of superoxide anion and hydrogen peroxide on Cav-1 is mediated through a transcription-independent mechanism that involves protein degradation via the ubiquitin-proteasome pathway. These results indicate the essential role of different ROS in cancer cell motility and through Cav-1 expression, which may provide a key mechanism controlling tumor progression and metastasis. The up-regulation of Cav-1 and cell motility by hydroxyl free radical suggests an important role of this ROS as a positive regulator of tumor progression.