Recombinant Membrane-targeted Form of CD59 Inhibits the Growth of Choroidal Neovascular Complex in Mice

• DOI: 10.1074/jbc.m110.153130
• Publication Date: 2010-08-25T00:14:14Z
• Authors: Nalini S. Bora; Purushottam Jha; Valeriy V. Lyzogubov; Sankaranarayanan ...; Juan Liu; Ruslana G. Tytarenko; Deborah A. Fraser; B. Paul Morgan; Puran S. Bora
• Keywords: CD59; Choroid

This study was designed to explore the effect of recombinant, membrane-targeted CD59 (rCD59-APT542) on growth and size fully developed neovascular complex using murine model laser-induced choroidal neovascularization (CNV). CNV induced by laser photocoagulation in C57BL/6 mice an argon laser, animals received rCD59-APT542 via intravitreal (ivt) route. Western blot analysis, immunohistochemistry, total complement hemolytic assay demonstrated that exogenously administered incorporated as well retained RPE choroid functionally active vivo. Single ivt injection during (i.e. at day 3 post-laser) resulted ∼79% inhibition further complex. The significantly (p < 0.05) reduced administration after has 7 post-laser). Treatment with blocked formation membrane attack (MAC), increased apoptosis decreased cell proliferation On basis results presented here we conclude recombinant targeted inhibited mouse model. We propose a combination two mechanisms: proliferation, both resulting from local MAC, may be responsible for rCD59-APT542.