Estrogen-related Receptor β Reduces the Subnuclear Mobility of Estrogen Receptor α and Suppresses Estrogen-dependent Cellular Function

• DOI: 10.1074/jbc.m114.619098
• Publication Date: 2015-03-25T02:55:24Z
• Authors: Takashi Tanida; Ken Ichi Matsuda; Shunji Yamada; Takashi Hashimoto; Mitsuhiro Kawata
• Keywords: Estrogen receptor beta; GPER; Estrogen-related receptor gamma

Estrogen-related receptor (ERR) is a member of the nuclear superfamily that has strong homology with estrogen (ER) α. ERR three subtypes (α, β, and γ) expressed in estrogen-sensitive organs, including ovary, breast, brain. No endogenous ligands ERRs have been identified, but these receptors share common DNA element ERα control estrogen-mediated gene transcription. Recent evidence suggests role estrogen-related pathophysiology, detailed mechanisms functions tissues are unclear. Using live-cell imaging fluorescent protein labeling, we found only ERRβ among exhibits punctate intranuclear pattern overlapping following 17β-estradiol (E2)-stimulation. Fluorescence recovery after photobleaching showed significant reduction mobility ligand-activated co-expression ERRβ. resonance energy transfer revealed directly interacts ERα. The N-terminal domain was identified as region We also correlation between cluster formation interaction receptors. Expression significantly repressed ERα-mediated transactivity, whereas other had no effect on transactivity Consistent this finding, E2-stimulated proliferation MCF-7 breast carcinoma cells bcl-2 expression inhibited by These results provide for suppressive signaling through findings further suggest unique inhibitory estrogen-dependent cellular function such cancer cell proliferation.