Lipogenesis Is Decreased by Grape Seed Proanthocyanidins According to Liver Proteomics of Rats Fed a High Fat Diet

• DOI: 10.1074/mcp.m000055-mcp201
• Publication Date: 2010-03-24T00:14:03Z
• Authors: Isabel Baiges; Johan Palmfeldt; Cinta Bladé; Niels Gregersen; Lluís Arola
• Keywords: Lipogenesis; Dyslipidemia; Proteome

Bioactive proanthocyanidins have been reported to several beneficial effects on health in relation metabolic syndrome, type 2 diabetes, and cardiovascular disease. We studied the effect of grape seed proanthocyanidin extract (GSPE) rats fed a high fat diet (HFD). This is first study flavonoids liver proteome suffering from syndrome. Three groups were over period 13 weeks either chow (control), an HFD, or supplemented for last 10 days with GSPE (HFD + GSPE). The was fractionated, using Triton X-114-based two-phase separation, into soluble membrane protein fractions so that total coverage considerably improved. data isobaric tag relative absolute quantitation (iTRAQ)-based nano-LC-MS/MS analysis revealed 90 proteins significant (p < 0.05) minimal expression difference 20% due syndrome versus control) 75 treatment HFD). same animals previously (Quesada, H., del Bas, J. M., Pajuelo, D., Díaz, S., Fernandez-Larrea, J., Pinent, Arola, L., Salvadó, M. Bladé, C. (2009) Grape correct dyslipidemia associated high-fat repress genes controlling lipogenesis VLDL assembling liver. Int. Obes. 33, 1007-1012), shown observed HFD-fed probably through repression hepatic lipogenesis. Our corroborate those findings extensive list describing induction glycogenesis, glycolysis, fatty acid triglyceride synthesis whereas opposite pattern large extent GSPE-treated animals. wider than thought, putative targets involved reversal symptoms revealed. Some these novel candidate such as GFPT1, CD36, PLAA (phospholipase A(2)-activating protein), METTL7B, SLC30A1, G signaling proteins, sulfide-metabolizing ETHE1 SQRDL (sulfide-quinone reductase-like) might be considered drug