Discovery and Verification of Osteopontin and Beta-2-microglobulin as Promising Markers for Staging Human African Trypanosomiasis

• DOI: 10.1074/mcp.m110.001008
• Publication Date: 2010-08-20T02:24:26Z
• Authors: Natalia Tiberti; Alexandre Hainard; Veerle Lejon; Xavier Robin; Dieudonné Mumba N...; Natacha Turck; Enock Matovu; John Enyaru; Joseph Mathu Ndung'u; Alexander Scherl; Loïc Dayon; Jean-Charles Sanchez
• Keywords: Beta-2 microglobulin; Tandem mass tag; Osteopontin

Human African trypanosomiasis, or sleeping sickness, is a parasitic disease endemic in sub-Saharan Africa, transmitted to humans through the bite of tsetse fly. The first hemolymphatic stage associated with presence parasites bloodstream, lymphatic system, and body tissues. If patients are left untreated, cross blood-brain barrier invade cerebrospinal fluid brain parenchyma, giving rise second meningoencephalitic stage. Stage determination crucial step guiding choice treatment, as drugs used for S2 potentially dangerous. Current staging methods, based on counting white blood cells demonstrating trypanosomes fluid, lack specificity and/or sensitivity. In present study, we several proteomic strategies discover new markers potential human trypanosomiasis. Cerebrospinal (CSF) samples were collected from infected Trypanosoma brucei gambiense Democratic Republic Congo. was determined following guidelines national control program. proteome analyzed by two-dimensional gel electrophoresis (n = 9), sixplex tandem mass tag (TMT) isobaric labeling 6) quantitative spectrometry. Overall, 73 proteins overexpressed presenting disease. Two these, osteopontin β-2-microglobulin, confirmed be trypanosomiasis (HAT) Western blot ELISA. two significantly discriminated between S1 high sensitivity (68% 78%, respectively) 100% specificity, combination both improved 91%. levels β-2-microglobulin CSF (μg/ml range), well fold increased concentration compared (3.8 5.5 make good candidates development test HAT patients.