Alteration of the Serum N-glycome of Mice Locally Exposed to High Doses of Ionizing Radiation

• DOI: 10.1074/mcp.m111.014639
• Publication Date: 2012-11-13T12:18:06Z
• Authors: Thibault Chaze; Marie-Christine S...; Fabien Milliat; Georges Tarlet; Tony Lefebvre-Dar...; Patrick Gourmelon; Eric Bey; Marc Benderitter; Jean-Claude Micha...; Olivier Guipaud
• Keywords: Glycome; Fucosylation; Proteome

Exposure of the skin to ionizing radiation leads characteristic reactions that will often turn into a pathophysiological process called cutaneous syndrome. The study this disorder is crucial finding diagnostic and prognostic bioindicators local exposure or effects. It known irradiation alters serum proteome content potentially post-translationally modifies proteins. In study, we investigated whether localized glycome. Two-dimensional differential in-gel electrophoresis proteins from man mice exposed showed potential post-translational modification changes occurred following irradiation. Using large-scale quantitative mass-spectrometry-based glycomic approach, performed global analysis glycan structures non-irradiated locally irradiated high doses γ-rays (20, 40, 80 Gy). Non-supervised descriptive statistical analyses (principal component analysis) using structure data allowed us discriminate between uninjured/slightly injured animals developed severe lesions. Decisional statistics several families were down-regulated whereas others increased, particular statistically significantly changed in mice. observed increases multiantennary N-glycans outer branch fucosylation sialylation associated with up-regulation genes involved glycosylation liver, which main producer proteins, an increase key proinflammatory cytokines IL-1β, IL-6, TNFα, can regulate expression genes. Our results suggest for first time role protein response These protein-associated might signal