Analysis of the Human Tissue-specific Expression by Genome-wide Integration of Transcriptomics and Antibody-based Proteomics

• DOI: 10.1074/mcp.m113.035600
• Publication Date: 2013-12-06T02:30:58Z
• Authors: Linn Fagerberg; Björn M. Hallström; Per Oksvold; Caroline Kampf; Dijana Djureinovic; Jacob Odeberg; Masato Habuka; Simin Tahmasebpoor; Angelika Danielsson; Karolina Edlund; Anna Asplund; Evelina Sjöstedt; Emma Lundberg; Cristina Al-Khali...; Marie Skogs; Jenny Ottosson Ta...; Holger Berling; Hanna Tegel; Jan Mulder; Peter Nilsson; Jochen M. Schwenk; Cecilia Lindskog; Frida Danielsson; Adil Mardinoglu; Åsa Sivertsson; Kalle von Feilitzen; Mattias Forsberg; Martin Zwahlen; IngMarie Olsson; Sanjay Navani; Mikael Huss; Jens Nielsen; Fredrik Ponten; Mathias Uhlén
• Keywords: Male; Proteomics; Proteome; Gene Expression; Biochemistry; Antibodies; transcriptomics; 03 medical and health sciences; proteomics; Humans; Gene Regulatory Networks; human; protein expression; Biokemi; Molecular Biology; 0303 health sciences; Molekylärbiologi; Gene Expression Profiling; article; Proteins; Genomics; human tissue; spermatogenesis; 3. Good health; Systems Integration; priority journal; protein analysis; Organ Specificity; Tissue Array Analysis; immunohistochemistry; gene expression; Female; Transcriptome

Global classification of the human proteins with regards to spatial expression patterns across organs and tissues is important for studies of human biology and disease. Here, we used a quantitative transcriptomics analysis (RNA-Seq) to classify the tissue-specific expression of genes across a representative set of all major human organs and tissues and combined this analysis with antibody-based profiling of the same tissues. To present the data, we launch a new version of the Human Protein Atlas that integrates RNA and protein expression data corresponding to ∼80% of the human protein-coding genes with access to the primary data for both the RNA and the protein analysis on an individual gene level. We present a classification of all human protein-coding genes with regards to tissue-specificity and spatial expression pattern. The integrative human expression map can be used as a starting point to explore the molecular constituents of the human body.