Proteomic Studies of the Singapore Grouper Iridovirus

• DOI: 10.1074/mcp.m500149-mcp200
• Publication Date: 2005-11-02T01:12:46Z
• Authors: Wenjun Song; Qingsong Lin; Shashikant B. Joshi; Teck Kwang Lim; Choy-Leong Hew
• Keywords: Iridovirus

The Singapore grouper iridovirus (SGIV) genome consists of a double-stranded circular DNA 140,131 base pairs with 162 predicted open reading frames. Our earlier study using peptide mass fingerprints generated from MALDI-TOF MS led to the identification 26 viral proteins. present investigation aimed achieve more comprehensive and precise SGIV proteome by two workflows: one-dimensional gel electrophoresis (1-DE) separation followed protein MALDI-TOF/TOF MS/MS (1-DE-MALDI workflow) shotgun proteomics in which whole virus was digested trypsin resulting peptides were separated nano-LC analyzed (LC-MALDI workflow). In total, 44 proteins identified, 25 reported for first time. Fourteen uniquely identified 1-DE-MALDI workflow, whereas another 10 only LC-MALDI workflow 20 found both approaches. Moreover 13 have acetylated N termini. Twenty-three contain transmembrane domains, accounting 52.3% total identified. RT-PCR confirmed transcription products all A large number workflows this significantly enhanced coverage proteome. is at iridoviral that has been extensively characterized. results should provide further insights into biology other iridoviruses.