Proteome Analysis of Hepatocellular Carcinoma by Two-dimensional Difference Gel Electrophoresis

• DOI: 10.1074/mcp.m600449-mcp200
• Publication Date: 2007-07-13T00:13:55Z
• Authors: Wei Sun; Baocai Xing; Yi Sun; Xiaojuan Du; Min Lu; Chunyi Hao; Zhuang Lu; Wei Mi; Songfeng Wu; Handong Wei; Xue Gao; Yunping Zhu; Ying Jiang; Xiaohong Qian; Fuchu He
• Keywords: Proteome; Hepatocellular adenoma; Prohibitin; Difference gel electrophoresis

Hepatocellular carcinoma (HCC) is a highly malignant tumor, and chronic infection with hepatitis B virus one of its major risk factors. To identify the proteins involved in HCC carcinogenesis, we used two-dimensional fluorescence DIGE to study differentially expressed tumor adjacent nontumor tissue samples. Samples from 12 virus-associated patients were analyzed. A total 61 spots significantly up-regulated (ratio ≥ 2, p ≤ 0.01) samples, whereas 158 down-regulated −2, 0.01). Seventy-one gene products identified among these spots. Members heat shock protein 70 90 families simultaneously up-regulated, metabolism-associated decreased The down-regulation mitochondrial peroxisomal results suggested loss special organelle functions during carcinogenesis. Four metabolic enzymes methylation cycle liver tissues, indicating S-adenosylmethionine deficiency HCC. Two products, glyceraldehyde-3-phosphate dehydrogenase formimidoyltransferase-cyclodeaminase, inversely altered spots, suggesting that different isoforms or post-translational modifications two might play roles For first time, overexpression Hcp70/Hsp90-organizing heterogeneous nuclear ribonucleoproteins C1/C2 tissues was confirmed by Western blot then immunohistochemistry staining their potential as markers. In summary, profiled proteome alterations may provide useful insights for understanding mechanism process Proteomics analysis currently considered be powerful tool global evaluation expression, proteomics has been widely applied diseases, especially fields cancer research. Quantitative expression profiling crucial part proteomics, such requires methods are able efficiently accurate reproducible differential values more biological Two-dimensional electrophoresis (2DE) 1The abbreviations are: 2DE, electrophoresis; 2D, two-dimensional; HOP, protein; HCC, hepatocellular carcinoma; HBV, virus; HCV, C hnRNP, ribonucleoprotein; TEMED, N,N,N′,N′-tetramethylethylenediamine; GAPDH, dehydrogenase; MAT, methionine adenosyltransferase; GNMT, glycine N-methyltransferase; BHMT, betaine-homocysteine S-methyltransferase; COMT, catechol O-methyltransferase; FTCD, formimidoyltransferase-cyclodeaminase; HSP, AdoMet, S-adenosylmethionine; GRP, glucose-regulated protein. technique However, intergel variation excessive time/labor costs have common problems standard 2DE. (2D) therefore most significant advances quantitative proteomics. 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