Integration of DIGE and Bioinformatics Analyses Reveals a Role of the Antiobesity Agent Tungstate in Redox and Energy Homeostasis Pathways in Brown Adipose Tissue

• DOI: 10.1074/mcp.m700198-mcp200
• Publication Date: 2007-11-07T01:13:19Z
• Authors: Sílvia Barceló-Ba...; Susana G. Kalko; Yaiza Esteban; Sílvia Moreno; María C. Carmona; Ramon Gomis
• Keywords: Tungstate; Lipid Oxidation; Thermogenin

Our previous results demonstrated that tungstate decreased weight gain and adiposity in obese rats through increased thermogenesis lipid oxidation, suggesting brown adipose tissue was one of the targets its antiobesity effect. To identify potential tungstate, we used DIGE to compare protein extracts from following experimental groups: untreated lean, tungstate-treated obese, rats. distinguish direct action those are secondary body loss, also included analysis an additional group consisting lose by caloric restriction. Hierarchical clustering variance t test contrasts clearly separated different groups. identified 20 proteins as obesity involved Krebs cycle, glycolysis, lipolysis fatty acid electron transport, redox. Protein oxidation treatment, confirming a role redox processes; however, palmitate measure beta-oxidation, not altered thus questioning putative function oxidation. network analyses using Ingenuity Pathways Analysis highlighted peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1alpha) target. We confirmed real time PCR indeed up-regulates PGC-1alpha, major target, uncoupling 1, shown Western blot. These illustrate utility proteomics bioinformatics approaches therapies suggest modulates processes increases energy dissipation PGC-1alpha up-regulation, contributing overall