Involvement of Acidic Fibroblast Growth Factor in Spinal Cord Injury Repair Processes Revealed by a Proteomics Approach

• DOI: 10.1074/mcp.m800076-mcp200
• Publication Date: 2008-05-16T00:12:43Z
• Authors: Ming-Chu Tsai; Li-Fen Shen; Huai-Sheng Kuo; Henrich Cheng; Kin-Fu Chak
• Keywords: Proteomics; 0303 health sciences; Blotting, Western; Proteins; Nerve Regeneration; Rats; Rats, Sprague-Dawley; 03 medical and health sciences; GAP-43 Protein; Spinal Cord; Protein Biosynthesis; Animals; Fibroblast Growth Factor 1; Stathmin; Female; Protein Processing, Post-Translational; Spinal Cord Injuries

Acidic fibroblast growth factor (aFGF; also known as FGF-1) is a potent neurotrophic that affects neuronal survival in the injured spinal cord. However, pathological changes occur with cord injury (SCI) and attribution to aFGF of neuroprotective effect during SCI are still elusive. In this study, we demonstrated rat SCI, when treated aFGF, showed significant functional recovery indicated by Basso, Beattie, Bresnahan locomotor rating scale combined behavior score (p < 0.01-0.001). Furthermore proteomics bioinformatics approaches were adapted investigate global protein profile damaged tissue experimental rats either or without at 24 h after injury. We found 51 spots, resolvable two-dimensional PAGE, had differential expression. Using hierarchical clustering analysis, these proteins categorized into five major expression patterns. Noticeably involved process secondary injury, such astrocyte activation (glial fibrillary acidic protein), inflammation (S100B), scar formation (keratan sulfate proteoglycan lumican), which lead blocking regeneration, down-regulated contusive treatment aFGF. propose might initiate series biological processes prevent attenuate this, turn, leads an improvement recovery. Moreover quantitative level was verified real time PCR. identified various potential factors induced may be repair rats. Thus, our results could have remarkable impact on clinical developments area therapy.