The Unique Features of Proteins Depicting the Chicken Amniotic Fluid

• DOI: 10.1074/mcp.ra117.000459
• Publication Date: 2018-02-15T01:55:14Z
• Authors: Mylène Da Silva; Clara Dombre; Aurélien Brionne; Philippe Monget; Magali Chessé; Marion De Pauw; Maryse Mills; Lucie Combes-Soia; Valérie Labas; Nicolas Guyot; Yves Nys; Sophie Réhault-Go...
• Keywords: Proteome; Lactoferrin

In many amniotes, the amniotic fluid is depicted as a dynamic milieu that participates in protection of embryo (cushioning, hydration, and immunity). However, birds, protein profile remains unexplored, even though its proteomic signature predicted to differ compared with humans. fact, unlike humans, chicken does not collect excretory products composition strikingly changes at mid-development because massive inflow egg white proteins, which are thereafter swallowed by support growth. Using GeLC-MS/MS shotgun strategies, we identified 91 nonredundant proteins delineating proteome day 11 development, before transfer. These were essentially associated metabolism nutrients, immune response developmental processes. Forty-eight common both human fluids, including serum albumin, apolipoprotein A1 alpha-fetoprotein. We further investigated effective role innate defense revealed it exhibits significant antibacterial activity development. This potential drastically enhanced after transfer, presumably due lysozyme, avian beta-defensin 11, vitelline membrane outer layer 1, beta-microseminoprotein-like most likely candidates. Interestingly, several recovered prior transfer uniquely found birds (ovalbumin related X Y, 11) or oviparous species (vitellogenins 1 2, riboflavin-binding protein). study provides an integrative overview opens stimulating perspectives deciphering egg-specific embryonic immunity. may constitute valuable biomarkers for poultry production detect hazardous situations (stress, infection, etc.), negatively affect development embryo.