Quantitative Proteomics of Strong and Weak Biofilm Formers of Enterococcus faecalis Reveals Novel Regulators of Biofilm Formation

• DOI: 10.1074/mcp.ra117.000461
• Publication Date: 2018-01-23T01:20:16Z
• Authors: Tanujaa Suriyanar...; Lin Qingsong; Lim Teck Kwang; Lee Yew Mun; Thuyen Truong; Chaminda Jayampat...
• Keywords: Enterococcus faecalis

Enterococcus faecalis is a bacterial pathogen associated with both endodontic and systemic infections. The biofilm formation ability of E. plays key role in its virulence drug resistance attributes. biofilms on implanted medical devices often results treatment failure. In the present study, we report protein markers using iTRAQ-based quantitative proteomics approach. order to elucidate biofilm-associated markers, investigated proteome strong weak biofilm-forming clinical isolates comparison standard American Type Culture Collection (ATCC) control strains. Comparison ATCC strains showed that proteins shikimate kinase pathway sulfate transport were up-regulated former, while secondary metabolites, cofactor biosynthesis, tetrahydrofolate biosynthesis down-regulated. nucleoside nucleotide up-regulated, whereas sugar Further gene ontology analyses revealed major differences arise from metabolic activity levels formers, higher observed former. could therefore be determinant new identified this study can further characterized understand their exact ability. This, turn, lead numerous therapeutic benefits oral pathogen. data has been deposited ProteomeXchange identifier PXD006542.