Endothelin receptor A is expressed and mediates the [Ca2+]i mobilization of cells in human ciliary smooth muscle, ciliary nonpigmented epithelium, and trabecular meshwork

• DOI: 10.1076/ceyr.17.1.31.5256
• Publication Date: 2003-02-13T14:08:14Z
• Authors: Wenhong Tao; Ganesh Prasanna; Slobodan Dimitrij...; Thomas Yorio
• Keywords: Ciliary muscle

PURPOSE. To identify which endothelin receptor subtype is expressed and functional in the human ciliary body trabecular meshwork, tissues that regulate aqueous humor dynamics. METHODS. Immunocytochemistry was used to characterize primary culture cells of normal ocular cells. Endothelin gene expression probed with reverse transcription polymerase chain reaction (RT-PCR). Intracellular calcium ([Ca 2+ ] i) mobilization measured video image microscopy using Fura-2AM as a fluorescent probe. RESULTS. Identities cultures, smooth muscle (HCSM), nonpigmented epithelial (HCE), meshwork (HTM) were confirmed by immuno-cytochemistry, cell-specific markers observing typical cell morphologies. The presence A (ET A) detected RT-PCR all three types mRNA phenotype verified restriction enzyme BamHI digestion. No ET B under conditions used. [Ca i HCSM increased from 57 ± 7 nM 328 108 (n = 23; mean SE; P < 0.05) 1 endothelin-1 (ET-1). In HCE cells, 40 3 90 10 55) (P 0.001) same concentration ET-1. Similarly, ET-1 (1 nM) 51 6 185 47 19) HTM agonist for B, S6c, had no effect on transients types. these experimental conditions. CONCLUSION. possibly responsible mediating signal muscle,