The effect of selective cyclooxygenase-2 inhibitor on corneal angiogenesis in the rat

• DOI: 10.1076/ceyr.19.4.300.5301
• Publication Date: 2003-02-13T14:02:59Z
• Authors: Masakazu Yamada; Masataka Kawai; Yuka Kawai; Yukihiko Mashima
• Keywords: Corneal Neovascularization

Eicosanoids that are present in inflamed tissues thought to play a significant role angiogenesis. Cyclooxygenase, key enzyme eicosanoid synthesis, has recently been shown exist two isoforms: the constitutive COX-1 and inducible COX-2. This study was undertaken determine of COX-2 corneal angiogenic response.Angiogenesis rat cornea provoked by chemical cautery. Either NS-398, selective inhibitor, or indomethacin, non-selective COX applied topically 3 times daily for 4 days. Neovascularization quantitated digital image analysis flat preparations. To test their inhibitory effects on normal cauterized corneas were incubated culture medium with inhibitor. Prostaglandin E2 assayed using an enzyme-linked immunosorbent assay.Both NS-398 indomethacin significantly inhibited neovascularization % inhibition 36.4 +/- 9.6%, 38.5 9.0%, respectively, when at concentration 0.1% (p <.001). Neither reduced response 0.01% below. PGE(2) production 2.0 higher than controls. In corneas, synthesis 80%, whereas it no more 20%. contrast, injured both similar fashion, maximal rate 75 80%.Our results suggest induction increases level eicosanoids, which result