The neuroprotective effect of 1,25-dyhydroxyvitamin D 3 (calcitriol) and probiotics on the rotenone-induced neurotoxicity model in SH-SY5Y cells

• DOI: 10.1080/01480545.2024.2429621
• Publication Date: 2024-11-25T08:37:08Z
• Authors: Fatma Hazan Gul; Nuh Mehmet Bozkurt; Nalan Hakime Nogay; Gokhan Unal

This study aimed to investigate the neuroprotective role of probiotics and 1,25-dyhydroxyvitamin D3(calcitriol) against neurotoxicity on rotenone-induced human neuroblastoma cell line sh-sY5Y. Rotenonewas administered to induce neurotoxic effects in sh-sY5Y cells. calcitriol and probiotics were administeredat different concentrations as pre- and post-treatment. the thiazolyl blue tetrazolium bromide (Mtt)assay was performed to measure cell viability. intracellular protein levels of antioxidant enzymes (proteintyrosine kinase (PtK), superoxide dismutase (sOD), glutathione peroxidase (Gsh), glutathione reductase(GsR), and catalase (cat)) were determined by the enzyme-linked immunosorbent assay (elisa).Rotenone (150 nM) reduced (p < 0.001) cell viability compared to control cells. single and combinedpretreatments with probiotics (0.01 mg/ml, 0.05 mg/ml, and 0.1 mg/ml) and calcitriol (1.25 µM, 2.5 µM,and 5 µM) increased (p < 0.05) cell viability compared to rotenone group. in the pre- and post-treatmentdesign, all treatment groups increased the sOD and Gsh levels and decreased the GsR levels comparedto rotenone. None of the pretreatments reversed the PtK levels (except probiotics: 0.01 mg/ml). calcitriol(2.5 µM) increased the cat levels in pretreatment design, and probiotics (0.05mg/ml and 0.1 mg/ml)increased cat levels in post-treatment design compared to rotenone group. calcitriol and probioticsprotect against rotenone-induced neurotoxicity in sh-sY5Y cells by decreasing reactive oxygen species(ROs) and increasing antioxidant enzyme parameters. these neuroprotective effects of calcitriol andprobiotics against rotenone-induced dopaminergic neurotoxicity provide an experimental basis for theirpotential clinical use in the treatment of Parkinson’s disease (PD).