Network pharmacology and in vivo experiment-based strategy to investigate mechanisms of JingFangFuZiLiZhong formula for ulcerative colitis

• DOI: 10.1080/07853890.2022.2095665
• Publication Date: 2022-11-16T10:34:00Z
• Authors: Mengyuan Wang; Jianan Li; Yuzhang Yin; Liying Liu; Yifei Wang; Ying Qu; Yanqiu Hong; Shuangshuang Ji; Tao Zhang; Nan Wang; Jinlong Liu; Xu Cao; Xiaobin Zao; Shuxin Zhang
• Keywords: Pharmacology; JingFangFuZiLiZhong formula; 0303 health sciences; R; bioinformatics; Network Pharmacology; Rats; 03 medical and health sciences; network pharmacology; Medicine; Humans; Animals; Colitis, Ulcerative; Intestinal Mucosa; ulcerative colitis; Signal Transduction

Background Ulcerative colitis (UC), a chronic inflammatory disease, often cause carcinogenesis, disability, and intestinal perforation. The JingFangFuZiLiZhong formula (JFFZLZ) shows good effect against UC in the clinic. Hence, we aim to investigate mechanisms between JFFZLZ via network pharmacology data mining vivo experiments.Methods We obtained active constituents related targets from public databases. overlapped genes were further analysed by enrichment analysis. hub used construct molecule docking finally screened out nine their expressions verified Gene Expression Omnibus database rats' colon tissues after treatment.Results results implied that mainly regulated signal transduction, metabolites production, inflammation pathways. expression of STAT3, CXCL8, IL6, CXCL12, TNF, TP53, PTPN11 both upregulated patients rats. While RELA, EGFR, TP53 downregulated patients, but Furthermore, could repair mucosal damage promote healing ulcers regulating targets.Conclusion These elucidated anti-UC was closely inhibition response, oxidative stress, repairing through different findings contribute better understanding regulation UC.Key messagesJFFZLZ reduce infiltration damage.Through pharmacology-based strategy mining, key pathways JFFZLF UC.The mechanism response stress targets.