Role of tRNA derived fragments in renal ischemia–reperfusion injury

• DOI: 10.1080/0886022x.2022.2072336
• Publication Date: 2022-05-12T05:13:36Z
• Authors: Dan Li; Hao Zhang; Xueqin Wu; Qing Dai; Shiqi Tang; Yan Liu; Shikun Yang; Wei Zhang
• Keywords: KEGG; Renal ischemia

Ischemia-reperfusion injury (IRI) is one of the major causes acute kidney (AKI). tRNA derived fragments (tRFs/tiRNAs) are groups small noncoding RNAs from tRNAs. To date, role tRFs/tiRNAs in renal IRI has not been reported. Herein, we aimed to investigate involvement occurrence and development ischemia-reperfusion-induced AKI.Moderate/severe mouse models were established by bilateral pedicle clamping. The tRF/tiRNA profiles healthy controls moderate/severe IRI-stressed tissues sequenced Illumina NextSeq 500. Candidate differentially expressed tiRNAs further verified RT-qPCR. Biological analysis was also performed.Overall, 152 moderate ischemic group compared with normal control (FC > 2, p < 0.05), which 47 upregulated 105 downregulated; severe group, 285 157 upregulated, 128 downregulated. RT-qPCR determination eight abundantly consistent sequencing results. Gene Ontology for target genes showed that most enriched cell components, molecular functions biological processes Golgi apparatus, cytoplasmic vesicles, protein binding, cellular localization multicellular organism development. Kyoto Encyclopedia Genes Genomes (KEGG) these mainly involved natural killer mediated cytotoxicity pathway, citrate cycle, regulation actin cytoskeleton signaling pathway.Our results indicated IRI. These may be effective partly via immunity, inflammation metabolism processes. genes, including tiRNA-Gly-GCC-003, tiRNA-Lys-CTT-003, tiRNA-His-GTG-002, might potential biomarkers therapeutic targets ischemia-reperfusion injury-induced injury.