Detection of beef adulteration by mitochondrial D-loop based species-specific polymerase chain reaction

• DOI: 10.1080/10942912.2017.1369103
• Publication Date: 2017-12-29T04:29:34Z
• Authors: Nagappa Karabasan...; P. S. Girish; Deepak Kumar; S. P. Singh
• Keywords: Species Identification; Beef Cattle

A species-specific polymerase chain reaction (PCR) was developed for the identification of beef that is cattle (Bos indicus) meat by targeting mitochondrial D-loop region. pair beef-specific primers designed to amplify conserved region and possibility cross-amplification ruled taking into account other species comprising mammals, birds, rodents fish. The amplified PCR product 381 bp evident in raw (n = 20), cooked (60, 80 100°C), autoclaved (121°C) micro-oven processed samples. Sensitivity assay at 0.1% detection adulteration limit (LOD) DNA 0.1 picograms (pg). Newly rapid sensitive hence could be applied meat.