Development and characterization of symbiotic microcapsules to enhance the viability of probiotic under stressed conditions

• DOI: 10.1080/10942912.2023.2254521
• Publication Date: 2023-09-28T16:41:56Z
• Authors: Afaf Ejaz; Muhmmad Afzaal; Farhan Saeed; Sadaf Waliat; Yasir Abbas Shah; Ali Imran; Noor Akram; Aasma Asghar; Huda Ateeq; Suliman Yousef Al...; Asad Nawaz; Degnet Teferi Asres
• Keywords: Nutrition. Foods and food supply; extrusion method; 04 agricultural and veterinary sciences; TP368-456; Food processing and manufacture; probiotics; encapsulation; TX341-641; gastrointestinal tract; Onion peels extract; 0405 other agricultural sciences; prebiotic activity

ABSTRACTIn the present research, the survival and sustainability of a Lactobacillus rhamnosus probiotic has been investigated with regard to the prebiotic impact of introducing two different kinds of onion (Allium cepa L.) peel extract to probiotic microcapsules. Effective inclusion of red and white onion peel extract featuring good prebiotic action into the microcapsules enhanced probiotic survival. The structure, distribution of size, zeta potential, and encapsulation efficacy of probiotics and substances in the extract were evaluated along with the probiotics capability to persist under simulated gastrointestinal circumstances. Fourier Transform Infrared Spectroscopy (FTIR) was employed to investigate the molecular structure and internal framework. The wall and core components possess adhesive relationships, as demonstrated by FTIR spectra. Probiotics that were free and those that were in capsules were evaluated as well in vitro in undesirable persistence performance (>90%). Probiotics with encapsulation exhibited substantially (p > .05) greater vitality compared free cells, in accordance with an in-vitro experiment. Under simulated gastrointestinal situations, free cells continued diminishing their vitality, but encapsulated cells maintained their viability count beyond the suggested level (107 cfu/g). SEM photographs indicated that probiotics have been effectively encapsulated inblends. The microcapsules were around 191 ± 2 and 176 ± 2 μm in size. These outcomes reveal that these kinds of microcapsules may encourage the probiotic L. rhamnosus prolonged viability and consistency under multiple conditions.