Upregulation of glyoxylate cycle genes uponParacoccidioides brasiliensisinternalization by murine macrophages andin vitronutritional stress condition

• DOI: 10.1080/13693780701670509
• Publication Date: 2008-03-06T16:43:03Z
• Authors: L. S. Derengowski; A. H. Tavares; S. Silva; L. S. Procópio; M. S. S. Felipe; I. Silva-Pereira
• Keywords: Regulon; Malate synthase

Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis, is a facultative intracellular human pathogen that can persist within macrophage phagolysosomes, indicating fungus has evolved defense mechanisms in order to survive under nutritionally poor environments. The analysis P. brasiliensis transcriptome revealed several virulence factor orthologs other microorganisms, including glyoxylate cycle genes. This allows utilization two-carbon (C2) compounds as carbon source gluconeogenesis. Semiquantitative RT-PCR analyses these genes were upregulated when was recovered from murine macrophages, without any additional vitro growth. induction this cycle, response microenvironments, shown be coordinated with upregulation gluconeogenic phosphoenolpyruvate carboxykinase gene. In addition, assays employing RNA extracted grown medium acetate instead glucose also showed increased levels transcripts. Our main results suggest uses an important adaptive metabolic pathway.