Circular RNA ITCH suppresses metastasis of gastric cancer via regulating miR-199a-5p/Klotho axis

• DOI: 10.1080/15384101.2021.1878327
• Publication Date: 2021-01-27T08:48:47Z
• Authors: Yan Wang; Huiting Wang; Ruiqi Zheng; Peipei Wu; Zixuan Sun; Jingyan Chen; Leilei Zhang; Chenxiao Zhang; Hui Qian; Jiajia Jiang; Wenrong Xu
• Keywords: Circular RNA; Competing Endogenous RNA

Circular RNAs (circRNAs) are considered as a new regulatory factor in growth, metastasis and therapeutic resistance of human cancers. But the clinical significance underlying mechanism circular RNA ITCH (circ-ITCH) gastric cancer (GC) remain unknown. In present study, we found that circ-ITCH was down-regulated GC cell lines, tissues their serum-derived exosomes. The level related to invasion depth. Functional assays showed overexpression inhibited proliferation, migration, epithelial mesenchymal transition (EMT) cells, whereas knockdown appeared an opposite effect. Bioinformatic analysis luciferase reporter assay confirmed acted miR-199a-5p sponge increased Klotho. expression miR-199-5p up-regulated negatively correlated with circ-ITCH. MiR-199a-5p mimics reversed effects on inhibiting induced by decreased Klotho cells. Our findings indicate suppresses acting increasing expression, which serves potential biomarker targets for diagnosis therapy GC.Abbreviations: CircRNAs: RNAs; GC: cancer; circ-ITCH: Itchy E3 ubiquitin protein ligase; ceRNA: competitive endogenous RNA; EMT: Epithelial–mesenchymal transition; siRNA: Small interfering TEM: transmission electron microscope; NTA: nanoparticle tracking analysis.