Detection of the thaumatin-like protein gene from Brassica rapa var. oleifera in honey with the Proofman-LMTIA method

• DOI: 10.1080/19476337.2024.2351912
• Publication Date: 2024-05-24T12:45:07Z
• Authors: Wei Yao; Dandan Xu; Dan Zhou; Jinxin Liu; Xiaohua Zhang; Yao Wang; Deguo Wang
• Keywords: Brassica rapa; Thaumatin; Camellia oleifera

Honey adulteration has been on the rise with increasing demand for high-quality honey. The ladders-shape melting temperature isothermal amplification (LMTIA) was a newly developed nucleic acid technique, and aim of this study to establish method combining LMTIA proofreading enzyme-mediated probe cleavage (Proof-man probe) detecting thaumatin-like protein (TLP) genes Brassica rapa var. oleifera in primers selected as target be designed, Proof-man reaction optimized, specificity, sensitivity, applicability honey samples were determined. results showed that highly specific detection sensitivity 30-40 copies genomic DNA/reaction at optimal 60°C, could completed within 20 min. This can detect low-price B. into high-price