Up-regulation of caveolin 1 mediated by chitosan activates Wnt/ β-catenin pathway in chronic refractory wound diabetic rat model

• DOI: 10.1080/21655979.2021.2017625
• Publication Date: 2022-01-09T04:43:11Z
• Authors: Jie Gao; Jiayuan Zhang; Lianheng Xia; Xuewei Liang; Wukun Ding; Meiyu Song; Linggen Li; Zhen Jia
• Keywords: Caveolin 1; Refractory (planetary science); Caveolin

Diabetes mellitus (DM) can be implicated in the perturbations of vascular integrity and dysfunction angiogenesis. Chitosan has advantage promoting endothelial cell proliferation. However, molecular mechanism action promotion wound healing by chitosan derivatives is still debated. In current study, DM with chronic (CW) model rats were prepared treated chitosan. Vascular cells isolated from granulation tissues conducted RNA sequencing. Two thousand three hundred sixteen genes up-regulated, while 1,864 down-regulated after treatment compared to CW group. Here, we observed that caveolin 1 (CAV1) was highly expressed induced Furthermore, CAV1 knockdown could compromise activation Wnt pathway reduction β-catenin rat aortic (RAOECs) brain endothelium four (RBE4s). Moreover, determined a direct interaction between IP assay. The C-terminus (24 586 amino acids) contributed these two proteins. Finally, protein docking analysis indicated fragments (253-261 'FYAITTLHN' 292-303 'KFLAITTDCLQI') might have affected structure facilitated resistance degradation. Taken together, our study demonstrates up-regulate expression, interact for canonical signaling activity. Our results deepens beneficial developing new targets assist enhancing pharmacological effect on angiogenesis against DM.