Novel carbon dots derived from Schizonepetae Herba Carbonisata and investigation of their haemostatic efficacy

• DOI: 10.1080/21691401.2017.1379015
• Publication Date: 2017-09-19T11:19:13Z
• Authors: Meiling Zhang; Yan Zhao; Jinjun Cheng; Xiaoman Liu; Yongzhi Wang; Xin Yan; Yue Zhang; Fang Lu; Qingguo Wang; Huihua Qu
• Keywords: Male; 0301 basic medicine; Mice; 03 medical and health sciences; Bleeding Time; Animals; Hemorrhage; Hemostatics; Drugs, Chinese Herbal; Nanostructures; 3. Good health

Schizonepetae Herba Carbonisata (SHC) has been used in traditional Chinese medicine (TCM) to treat haemorrhagic diseases for more than 1000 years. However, little information is available on its haemostatic components and mechanism. In this study, we developed novel water-soluble carbon dots (CDs) aqueous extracts of SHC the first time a modified pyrolysis method was prepare using (SH) as sole precursor. The SHC-CDs were characterized transmission electron microscopy (TEM), high-resolution TEM (HRTEM), Fourier transform infrared (FT-IR), ultraviolet–visible (UV–Vis) fluorescence spectroscopy, X-ray photoelectron spectroscopy (XPS), diffraction (XRD) high-performance liquid chromatography (HPLC). Furthermore, CDs with quantum yield (QY) around 2.26% exhibited no toxicity within approximately 0.84 mg/mL CCK-8 assay. More interestingly, tail haemorrhaging liver experiments showed that CDs-treated mice had significantly shorter bleeding did normal saline (NS)-treated control group. Coagulation assays suggested could stimulate extrinsic blood coagulation system activate fibrinogen system. These results possess remarkable property, which provides evidence support further investigation considerable potential effective material basis TCM.