Quantitation of an alpha subunit splicing intermediate: evidence for transcriptional activation in the control of acetylcholine receptor expression in denervated chick skeletal muscle.

• DOI: 10.1083/jcb.104.5.1337
• Publication Date: 2004-05-15T00:18:20Z
• Authors: B H Shieh; M Ballivet; J Schmidt
• Keywords: Mature messenger RNA

We have investigated the mechanisms responsible for increase in acetylcholine receptor subunit mRNAs during induction of denervation supersensitivity skeletal muscle. Using a cRNA probe specific exon 7 (224 nucleotides; with flanking intron sequences 105 nucleotides on 3' end, and 70 5' end) alpha chicken muscle gene, we were able to quantitate concentration mature mRNA its precursor. In 3-wk-old chicks, message leg was found be 4.0 attomoles per microgram total RNA, 40-fold within 1 wk after section sciatic nerve. The molar ratio precursor/mature mRNA, which approximately 0.023 innervated as well denervated muscle, transiently rose 0.047 at beginning second postoperative day when content increased 20-fold; rise precursor level preceded content. These findings suggest that an accelerated rate transcription coding causes stimulation synthesis characteristic