The axonally secreted protein axonin-1 is a potent substratum for neurite growth.

• DOI: 10.1083/jcb.112.3.449
• Publication Date: 2004-05-15T00:18:20Z
• Authors: E T Stoeckli; T B Kuhn; C O Duc; M A Ruegg; P Sonderegger
• Keywords: Neurite; Growth cone; Membrane glycoproteins

Axonin-1 is a neuronal glycoprotein occurring both as membrane-bound and secreted form. Membrane-bound axonin-1 predominantly located in membranes of developing nerve fiber tracts has recently been characterized cell adhesion molecule; the soluble form from axons accumulates cerebrospinal fluid vitreous eye. In present study, we addressed question to whether was released functionally competent found that it strongly promotes neurite outgrowth when presented neurons an immobilized substratum. Neurite lengths elaborated by embryonic dorsal root ganglia on were similar those established neurite-promoting substrata L1 laminin. Fab fragments antibodies completely inhibited growth axonin-1, but not other substrata. form, had anti-adhesive effect, revealed perturbation fasciculation. view their structural similarity, conclude interact with same growth-promoting neuritic receptor. The fact active, together our previous findings internal cellular pool, suggests functional dualism between at site secretion, which most likely cone. secretion molecules could represent powerful rapidly acting regulatory element cone-neurite interactions control elongation, pathway selection, possibly target recognition.