Differential dynamics of neurofilament-H protein and neurofilament-L protein in neurons.

• DOI: 10.1083/jcb.127.1.173
• Publication Date: 2004-05-15T00:22:22Z
• Authors: S Takeda; S Okabe; T Funakoshi; N Hirokawa
• Keywords: Neurofilament; Immunoelectron microscopy; Photobleaching

Neurofilaments (NFs) are composed of triplet proteins, NF-H, NF-M, and NF-L. To understand the dynamics NFs in vivo, we studied NF-H compared them to those NF-L, using combination microinjection technique fluorescence recovery after photobleaching. In case NF-L protein, bleached zone gradually restored its intensity with a half time approximately 35 min. On other hand, was considerably faster, taking place 19 However, both cases stationary. Thus, it suggested that is dynamic component NF array interchangeable, but assembles neurofilament proteins more exchangeable way, implying location periphery core mainly subunits. Immunoelectron microscopy investigations incorporation sites labeled biotin compounds also revealed lateral insertion subunits into preexisting array, pattern seen summary, our results demonstrate L H subunit situ quite different from each other, suggesting separated mechanisms or structural specialization underlying behavior two proteins.