Integral Membrane Proteins of the Nuclear Envelope Are Dispersed throughout the Endoplasmic Reticulum during Mitosis

• DOI: 10.1083/jcb.137.6.1199
• Publication Date: 2002-07-26T16:46:23Z
• Authors: Li Yang; Tinglu Guan; Larry Gerace
• Keywords: Nuclear lamina; Immunogold labelling; Inner membrane; Nuclear pore; Nuclear membrane

We have analyzed the fate of several integral membrane proteins nuclear envelope during mitosis in cultured mammalian cells to determine whether are present a vesicle population distinct from bulk ER membranes after mitotic disassembly or dispersed throughout ER. Using immunofluorescence staining and confocal microscopy, we compared localization two inner (laminaassociated polypeptides 1 2 [LAP1 LAP2]) pore protein (gp210) distribution membranes, which was determined with lipid dyes (DiOC6 R6) polyclonal antibodies. found that at resolution this technique, three markers become completely mitosis. In agreement these results, detected LAP1 most containing by immunogold electron microscopy metaphase cells. Together, findings indicate lose their identity as subcompartment lamins begin reassemble around chromosomes end same time LAP2 propose reassembly involves sorting chromosome surfaces binding interactions chromatin.