A Novel Mammalian, Mitotic Spindle–associated Kinase Is Related to Yeast and Fly Chromosome Segregation Regulators

• DOI: 10.1083/jcb.138.3.643
• Publication Date: 2002-07-26T16:46:23Z
• Authors: Ganesan Gopalan; Clarence S.M. Chan; Peter J. Donovan
• Keywords: Aurora B kinase; Spindle pole body; Midbody; Polo-like kinase; Multipolar spindles; PLK1; Aurora inhibitor; Nocodazole; Mitotic exit; Telophase

We describe a novel mammalian protein kinase related to two newly identified yeast and fly kinases—Ipl1 aurora, respectively—mutations in which cause disruption of chromosome segregation. have designated this as Ipl1- aurora-related 1 (IAK1). IAK1 expression mouse fibroblasts is tightly regulated temporally spatially during the cell cycle. Transcripts first appear at G1/S boundary, are elevated M-phase, disappear rapidly after completion mitosis. The levels activity also cycle with peak M-phase. has distinct subcellular temporal pattern localization. It on centrosomes immediately duplicated separated. remains centrosome centrosome-proximal part spindle throughout mitosis detected weakly midbody microtubules telophase cytokinesis. In cells recovering from nocodazole treatment taxol-treated mitotic cells, associated microtubule organizing centers. A wild-type mutant form defects lethality ipl1 but not suggesting that interferes Ipl1p function yeast. Taken together, these data strongly suggest may an important role and/ or segregation cells. new member emerging subfamily serine/threonine superfamily. members be regulators