Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane

• DOI: 10.1083/jcb.200606017
• Publication Date: 2006-11-28T03:38:48Z
• Authors: Sabine Witzel; Vitaly Zimyanin; Filipa Carreira-B...; Masazumi Tada; Carl-Philipp Heis...
• Keywords: Dishevelled; Convergent extension; Cell polarity; Cell membrane

Wnt11 is a key signal, determining cell polarization and migration during vertebrate gastrulation. It known that functionally interacts with several signaling components, the homologues of which control planar polarity in Drosophila melanogaster. Although D. melanogaster these components are thought to polarize cells by asymmetrically localizing at plasma membrane, it not yet clear whether their subcellular localization plays similarly important role vertebrates. We show zebrafish embryonic cells, locally functions membrane accumulating its receptor, Frizzled 7, on adjacent sites contacts. Wnt11-induced 7 accumulations recruit intracellular Wnt mediator Dishevelled, as well itself, increase contact persistence. This persistence mediated local interaction Wnt11, atypical cadherin Flamingo does require activity further downstream effectors signaling, such RhoA Rok2. propose interacting Flamingo, modulates coordinate movements