New Tetrahymena basal body protein components identify basal body domain structure

• DOI: 10.1083/jcb.200703109
• Publication Date: 2007-09-05T00:54:41Z
• Authors: Chandra L. Kilburn; Chad G. Pearson; Edwin P. Romijn; Janet B. Meehl; Thomas H. Giddings; Brady P. Culver; John R. Yates; Mark Winey
• Keywords: Basal body; Basal (medicine); Proteome; Motile cilium; Organelle; Immunoelectron microscopy

Basal bodies organize the nine doublet microtubules found in cilia. Cilia are required for a variety of cellular functions, including motility and sensing stimuli. Understanding this biochemically complex organelle requires an inventory molecular components contribution each makes to overall structure. We define basal body proteome determine specific localization ciliated protozoan Tetrahymena thermophila. Using biochemical, bioinformatic, genetic approach, we identify 97 known candidate proteins. 24 novel T. thermophila proteins were identified, 19 which localized ultrastructural level, as seen by immunoelectron microscopy. Importantly, find from several structural domains within body, allowing us reveal how component contributes organization. Thus, present high resolution map structure highlighting important new future functional studies.