Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis

• DOI: 10.1083/jcb.201002035
• Publication Date: 2010-08-17T04:19:21Z
• Authors: Ioannis P. Nezis; Bhupendra V. Shra...; Antonia P. Sagona; Trond Lamark; Geir Bjørkøy; Terje Johansen; Tor Erik Rusten; Andreas Brech; Eric H. Baehrecke; Harald Stenmark
• Keywords: Nurse cell; Fragmentation; BAG3; Schneider 2 cells

Autophagy is an evolutionarily conserved pathway responsible for degradation of cytoplasmic material via the lysosome. Although autophagy has been reported to contribute cell death, underlying mechanisms remain largely unknown. In this study, we show that controls DNA fragmentation during late oogenesis in Drosophila melanogaster. Inhibition by genetically removing function genes atg1, atg13, and vps34 resulted stage egg chambers contained persisting nurse nuclei without fragmented attenuation caspase-3 cleavage. The inhibitor apoptosis (IAP) dBruce was found colocalize with autophagic marker GFP-Atg8a accumulated mutants. Nurse cells lacking Atg1 or Vps34 addition DNA. This indicates cells. Our results reveal IAP as a novel mechanism triggering death thereby provide mechanistic link between death.