Contractile forces regulate cell division in three-dimensional environments

• DOI: 10.1083/jcb.201309029
• Publication Date: 2014-04-28T15:53:25Z
• Authors: Ayelet Lesman; Jacob Notbohm; David A. Tirrell; Guruswami Ravicha...
• Keywords: Matrix (chemical analysis)

Physical forces direct the orientation of cell division axis for cells cultured on rigid, two-dimensional (2D) substrates. The extent to which physical regulate in three-dimensional (3D) environments is not known. Here, we combine live-cell imaging with digital volume correlation map 3D matrix displacements and identify sites at apply contractile force as they divide. Dividing embedded fibrous matrices remained anchored by long, thin protrusions. During rounding, released adhesive contacts near body while applying tensile tips protrusions axis. After cytokinesis, daughter respread into voids invaded maintaining traction persistent newly formed Mechanical interactions between extracellular constitute an important mechanism regulation environments.