A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells
0301 basic medicine
Time Factors
[SPI] Engineering Sciences [physics]
Transfection
Mechanotransduction, Cellular
Models, Biological
[PHYS] Physics [physics]
[SPI]Engineering Sciences [physics]
03 medical and health sciences
Cell Movement
Cell Line, Tumor
[CHIM] Chemical Sciences
Chlorocebus aethiops
Cell Adhesion
Pressure
[CHIM]Chemical Sciences
Animals
Humans
Actinin
Cell Shape
Research Articles
[PHYS]Physics [physics]
Focal Adhesions
Nonmuscle Myosin Type IIA
Actins
Actin Cytoskeleton
COS Cells
DOI:
10.1083/jcb.201311104
Publication Date:
2014-04-08T05:54:30Z
AUTHORS (12)
ABSTRACT
How adherent and contractile systems coordinate to promote cell shape changes is unclear. Here, we define a counterbalanced adhesion/contraction model for control. Live-cell microscopy data showed crucial role meshwork at the top of cell, which composed actin arcs myosin IIA filaments. The organized like muscle sarcomeres, with repeating II filaments separated by bundling protein α-actinin, mechanically coupled noncontractile dorsal fibers that run from bottom in cell. When contracts, it pulls away substrate. This pulling force fibers’ attachment focal adhesions, causing bend downward flattening likely be relevant understanding how cells configure themselves complex surfaces, protrude into tight spaces, generate three-dimensional forces on growth substrate under both healthy diseased conditions.
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CITATIONS (179)
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