Three distinct ribosome assemblies modulated by translation are the building blocks of polysomes

• DOI: 10.1083/jcb.201406040
• Publication Date: 2015-02-26T12:43:13Z
• Authors: Gabriella Viero; Lorenzo Lunelli; Andrea Passerini; Paolo Bianchini; Robert J. Gilbert; Paola Bernabò; Toma Tebaldi; Alberto Diaspro; Cecilia Pederzolli; Alessandro Quattrone
• Keywords: Polysome; Eukaryotic Ribosome; Ribosome profiling; Eukaryotic translation

Translation is increasingly recognized as a central control layer of gene expression in eukaryotic cells. The overall organization mRNA and ribosomes within polysomes, well the possible role this translation are poorly understood. Here we show that polysomes primarily formed by three distinct classes ribosome assemblies. We observe these assemblies can be connected naked RNA regions transcript. relative proportions reflect, probably dictate, level translational activity. These results reveal existence recurrent supra-ribosomal building blocks forming suggest presence unexplored controls embedded polysome structure.