Concerted actions of distinct nonmuscle myosin II isoforms drive intracellular membrane remodeling in live animals

• DOI: 10.1083/jcb.201612126
• Publication Date: 2017-06-09T14:15:15Z
• Authors: Oleg Milberg; Akiko Shitara; Seham Ebrahim; Andrius Masedunskas; Muhibullah Tora; Duy T. Tran; Yun Chen; Mary Anne Conti; Robert S. Adelstein; Kelly G. Ten Hagen; Roberto Weigert
• Keywords: Actin remodeling

Membrane remodeling plays a fundamental role during variety of biological events. However, the dynamics and molecular mechanisms regulating this process within cells in mammalian tissues situ remain largely unknown. In study, we use intravital subcellular microscopy live mice to study actomyosin cytoskeleton driving membranes large secretory granules, which are integrated into plasma membrane regulated exocytosis. We show that two isoforms nonmuscle myosin II, NMIIA NMIIB, control distinct steps integration process. Furthermore, find F-actin is not essential for recruitment NMII granules but key assembly activation contractile filaments. Our data support dual providing mechanical forces required remodel lipid bilayer serving as scaffold recruit regulatory molecules.