SLAMF1 is required for TLR4-mediated TRAM-TRIF–dependent signaling in human macrophages

• DOI: 10.1083/jcb.201707027
• Publication Date: 2018-02-13T16:24:53Z
• Authors: Maria Yurchenko; Astrid Skjesol; Liv Ryan; Gabriel Mary Richard; Richard Kumaran K...; Ninghai Wang; Cox Terhorst; Harald Husebye; Terje Espevik
• Keywords: TRIF

Signaling lymphocytic activation molecule family 1 (SLAMF1) is an Ig-like receptor and a costimulatory that initiates signal transduction networks in variety of immune cells. In this study, we report SLAMF1 required for Toll-like 4 (TLR4)-mediated induction interferon β (IFNβ) killing Gram-negative bacteria by human macrophages. We found controls trafficking the Toll receptor-associated (TRAM) from endocytic recycling compartment (ERC) to Escherichia coli phagosomes. resting macrophages, localized ERC, but upon addition E. coli, it trafficked together with TRAM ERC phagosomes Rab11-dependent manner. endogenous protein interacted defined key interaction domains as amino acids 68 95 well 15 C-terminal SLAMF1. Interestingly, SLAMF1-TRAM was observed not mouse proteins. Overall, our observations suggest new target modulation TLR4-TRAM-TRIF inflammatory signaling