RAB6 and microtubules restrict protein secretion to focal adhesions

• DOI: 10.1083/jcb.201805002
• Publication Date: 2019-05-29T19:44:31Z
• Authors: Lou Fourriere; Amal Kasri; Nelly Gareil; Sabine Bardin; Hugo Bousquet; David Pereira; Franck Perez; Bruno Goud; Gaelle Boncompain; Stéphanie Miserey...
• Keywords: Transport protein; Secretory protein

To ensure their homeostasis and sustain differentiated functions, cells continuously transport diverse cargos to various cell compartments in particular the surface. Secreted proteins are transported along intracellular routes from endoplasmic reticulum through Golgi complex before reaching plasma membrane microtubule tracks. Using a synchronized secretion assay, we report here that exocytosis does not occur randomly at surface but on localized hotspots juxtaposed focal adhesions. Although microtubules involved, RAB6-dependent machinery plays an essential role. We observed that, irrespective of cargos, most post-Golgi carriers positive for RAB6 its inactivation leads broad reduction protein secretion. may thus be general regulator