Cell cycle– and genomic distance–dependent dynamics of a discrete chromosomal region

• DOI: 10.1083/jcb.201807162
• Publication Date: 2019-03-07T19:54:12Z
• Authors: Hanhui Ma; Li-Chun Tu; Yu-Chieh Chung; Ardalan Naseri; David Grunwald; Shaojie Zhang; Thoru Pederson
• Keywords: Interphase; Dynamics

In contrast to the well-studied condensation and folding of chromosomes during mitosis, their dynamics interphase are less understood. We deployed a CRISPR-based DNA imaging system track genomic loci situated kilobases megabases apart on single chromosome. Two distinct modes were resolved: local movements as well ones that might reflect translational entire domain within nucleoplasmic space. The magnitude both these increased from early late G1, whereas reduced in S phase. fluctuations decreased slightly more markedly mid-late S. These newly observed cell cycle dependence suggest existence hitherto unrecognized compaction–relaxation dynamic chromosome fiber, operating concurrently with changes extent overall 4D genome.