Robust microtubule dynamics facilitate low-tension kinetochore detachment in metaphase

• DOI: 10.1083/jcb.202202085
• Publication Date: 2023-05-11T13:25:54Z
• Authors: Sneha Parmar; Samuel J. Gonzalez; Julia M. Heckel; Soumya Mukherjee; Mark McClellan; Duncan J. Clarke; Marnie Johansson; Damien Tank; Athena Geisness; David K. Wood; Melissa K. Gardner
• Keywords: Chromosome Segregation; Centromere; Saccharomycetales; Mitosis; Kinetochores; Microtubules; Article; Metaphase

During mitosis, sister chromatids are stretched apart at their centromeres via attachment to oppositely oriented kinetochore microtubules. This stretching generates inwardly directed tension across the separated centromeres. The cell leverages this signal detect and then correct potential errors in chromosome segregation, a mechanical signaling pathway that detaches improperly attached kinetochores from However, sequence of events leading up these detachment remains unknown. In study, we used microfluidics sustain observe low-tension budding yeast metaphase spindles over multiple hours, allowing us elucidate history prior event. We found that, under conditions which phosphorylation weakens kinetochore-microtubule connections, forces produced dynamic growth shortening microtubules is required efficiently facilitate events. Our findings underscore critical role robust microtubule dynamics ensuring fidelity segregation during mitosis.