Donor-type CD4+CD25+ Regulatory T Cells Suppress Lethal Acute Graft-Versus-Host Disease after Allogeneic Bone Marrow Transplantation

• DOI: 10.1084/jem.20020399
• Publication Date: 2002-09-30T17:03:17Z
• Authors: Petra Hoffmann; Joerg Ermann; Matthias Edinger; C. Garrison Fathman; Samuel Strober
• Keywords: Male; Mice, Inbred BALB C; Graft vs Host Disease; Receptors, Interleukin-2; Lymphocyte Activation; Article; Interleukin-10; 3. Good health; Mice, Inbred C57BL; Mice; 03 medical and health sciences; 0302 clinical medicine; T-Lymphocyte Subsets; Acute Disease; CD4 Antigens; Animals; Transplantation, Homologous; Lymphocyte Culture Test, Mixed; Bone Marrow Transplantation

Acute graft-versus-host disease (aGVHD) is still a major obstacle in clinical allogeneic bone marrow (BM) transplantation. CD4+CD25+ regulatory T (Treg) cells have recently been shown to suppress proliferative responses of CD4+CD25− alloantigenic stimulation vitro and are required for ex vivo tolerization donor cells, which results their reduced potential induce aGVHD. Here we show that isolated from the spleen or BM C57BL/6 (H-2b) mice not tolerized potent inhibitors alloresponse lethal aGVHD induced by irradiated BALB/c (H-2d) hosts vivo. The addition Treg at 1:1 ratio with responder/inducer resulted >90% inhibition mixed leukocyte reaction marked protection GVHD. This protective effect depended part on ability transferred secrete interleukin 10 occurred if were donor, but host, origin. Our demonstrate balance donor-type conventional can determine outcome