Regulation of IL-27 p28 gene expression in macrophages through MyD88- and interferon-γ–mediated pathways

• DOI: 10.1084/jem.20061440
• Publication Date: 2007-01-17T03:18:44Z
• Authors: Jianguo Liu; Xiuqin Guan; Xiaojing Ma
• Keywords: Lipopolysaccharides; Mice, Knockout; 0301 basic medicine; Binding Sites; DNA, Complementary; Base Sequence; Interleukins; Macrophages; Molecular Sequence Data; NF-kappa B; Articles; In Vitro Techniques; Mice, Inbred C57BL; Interferon-gamma; Mice; Protein Subunits; 03 medical and health sciences; Gene Expression Regulation; Myeloid Differentiation Factor 88; Animals; Female; Promoter Regions, Genetic; Interferon Regulatory Factor-1

Interleukin (IL)-27 is the newest member of IL-12 family heterodimeric cytokines composed Epstein-Barr virus–induced gene 3 and p28 chains. IL-27 not only plays an important role in regulation differentiation naive T helper cells but also possesses antiinflammatory properties. early product activated monocytes/macrophages dendritic cells. However, mechanisms whereby inflammatory signals stimulate production have been explored. In this study, we investigated transcriptional mouse macrophages response to lipopolysaccharide (LPS) interferon (IFN)-γ. We found that LPS-stimulated was completely dependent on Toll-like receptor 4/myeloid factor 88 (MyD88)–mediated pathway partially nuclear κB c-Rel. IFN-γ–induced production/secretion MyD88 independent then cloned promoter mapped its multiple transcription initiation sites. Furthermore, identified critical elements mediate inductive effects LPS IFN-γ, separately synergistically, a c-Rel– regulatory 1–dependent manner, respectively.