Peripheral CD103+ dendritic cells form a unified subset developmentally related to CD8α+ conventional dendritic cells

• DOI: 10.1084/jem.20091627
• Publication Date: 2010-03-30T03:21:29Z
• Authors: Brian T. Edelson; Wumesh KC; Richard Juang; Masako Kohyama; Loralyn A. Benoit; Paul A. Klekotka; Clara Moon; Jörn C. Albring; Wataru Ise; Drew G. Michael; Deepta Bhattacharya; Thaddeus S. Stapp...; Michael J. Holtzman; Sun-Sang J. Sung; Theresa L. Murphy; Kai Hildner; Kenneth M. Murphy
• Keywords: Mesenteric lymph nodes; Cross-Presentation; Priming (agriculture)

Although CD103-expressing dendritic cells (DCs) are widely present in nonlymphoid tissues, the transcription factors controlling their development and relationship to other DC subsets remain unclear. Mice lacking factor Batf3 have a defect of CD8α+ conventional DCs (cDCs) within lymphoid tissues. We demonstrate that Batf3−/− mice also lack CD103+CD11b− lung, intestine, mesenteric lymph nodes (MLNs), dermis, skin-draining nodes. Notably, displayed reduced priming CD8 T after pulmonary Sendai virus infection, with increased inflammation. In MLNs deficiency resulted specific DCs, population CD103+CD11b+ remaining intact. showed no evidence spontaneous gastrointestinal inflammation had normal contact hypersensitivity (CHS) response, despite previous suggestions CD103+ were required for immune homeostasis gut CHS. The between cDCs implied by shared dependence on was further supported similar patterns gene expression developmental Irf8. These data provide organ–resident DCs.