Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation

• DOI: 10.1084/jem.20162042
• Publication Date: 2018-02-12T15:07:33Z
• Authors: Jean-Marie Carpier; Andres E. Zucchetti; Laurence Bataille; Stéphanie Dogniaux; Massiullah Shafaq...; Sabine Bardin; Marco Lucchino; Mathieu Maurin; Leonel D. Joannas; Joao Gamelas Maga...; Ludger Johannes; Thierry Galli; Bruno Goud; Claire Hivroz
• Keywords: Immunological synapse

The adapter molecule linker for activation of T cells (LAT) orchestrates the formation signalosomes upon cell receptor (TCR) stimulation. LAT is present in different intracellular pools and dynamically recruited to immune synapse However, traffic its function lymphocyte are ill defined. We show herein that LAT, once internalized, transits through Golgi–trans-Golgi network (TGN), where it repolarized synapse. This retrograde transport depends on small GTPase Rab6 target soluble N-ethylmaleimide-sensitive factor attachment protein (t-SNARE) Syntaxin-16, two regulators endosome-to-Golgi/TGN transport. also vitro Syntaxin-16– or Rab6-silenced human vivo CD4+ lymphocytes knockout mouse this controls TCR These results establish from plasma membrane Golgi-TGN polarized delivery at activation.