Lack of evidence for participation of TMEM150C in sensory mechanotransduction

• DOI: 10.1085/jgp.202213098
• Publication Date: 2022-10-18T19:02:13Z
• Authors: Julia Ojeda-Alonso; Valérie Bégay; Jonathan Alexis G...; Andrea Fernanda C...; Bettina Purfürst; Gary R. Lewin
• Keywords: Mechanotransduction

The membrane protein TMEM150C has been proposed to form a mechanosensitive ion channel that is required for normal proprioceptor function. Here, we examined whether expression of in neuroblastoma cells lacking Piezo1 associated with the appearance currents. Using three different modes mechanical stimuli, indentation, stretch, and substrate deflection, could not evoke currents expressing TMEM150C. We next asked if necessary mechanosensitivity cutaneous sensory neurons. used an available mouse model which Tmem150c locus was disrupted through insertion LacZ cassette splice acceptor should lead transcript truncation. Analysis these mice indicated ablation gene complete neurons dorsal root ganglia (DRG). CRISPR/Cas9 strategy, made second large part deleted established Tmem150c-/- completely lack DRGs. ex vivo skin nerve preparation characterize mechanosenstivity mechanoreceptors nociceptors glabrous mice. found no quantitative alterations physiological properties any type fiber Since it claimed function, analysis locomotion Here again, indication there altered gait compared wild-type controls. In summary, conclude existing models have investigate function are problematic. Furthermore, find evidence forms or