Preparation of bimetallic calcium chromatite nanocomposite using Stevia rebaudiana leaf extract for inducing apoptosis in human cancer cell lines

• DOI: 10.1088/1402-4896/ad8aa7
• Publication Date: 2024-10-23T22:55:57Z
• Authors: Sitah Fahad Alanazi; Chithra Sivanandan
• Keywords: Stevia rebaudiana; Bimetallic strip; Cancer cell lines

Abstract Despite the widespread industrial use of chromium-based compounds, there are concerns regarding their toxicity in biomedical applications. Herein, bimetallic calcium chromatite nanocomposite (CC) was prepared through green synthesis using Stevia rebaudiana leaf extract. A porous nanorod CC obtained calcinations at 800oC. The formation confirmed by SEM (scanning electron microscopy), EDAX (Energy-dispersive X-ray spectroscopy), HRTEM (High-resolution transmission TGA (Thermal gravimetric analysis), and BET (Brunauer-Emmett-Teller). cytotoxicity evaluated MTT method against cancer lines (HeLa, U87, A549) primary cell line (HUVEC). IC50 value lower than lines. calculated specificity index greater one, indicating that this might target cells without a targeting moiety. Gene expression studies revealed upregulated proapoptotic gene, BAX downregulated anti-apoptotic Bcl-2, to induce apoptosis. KDR VEGF genes responsible for angiogenesis were down-regulated, decreases promote findings suggest phytoextract could be considered an adjuvant therapy treatment.