Microfluidics Microbial Activity MicroAssay: An Automated In Situ Microbial Metabolic Detection System

• DOI: 10.1089/ast.2021.0072
• Publication Date: 2022-01-20T15:05:27Z
• Authors: David Touchette; Ianina Altshuler; Isabelle Raymond-...; Miguel Ángel Fern...; Louis-Jacques Bou...; Brady O'Connor; Antonio J. Ricco; Lyle G. Whyte
• Keywords: Metabolic activity

With no direct extant-life detection instrumentation included in a space mission since the 1970s, advancement of new technologies to be future missions is imperative. We developed, optimized, and tested semi-automated prototype, microfluidics Microbial Activity MicroAssay (μMAMA). This system metabolically characterizes detects extant microbial life by way metabolism-indicator redox dyes. first evaluated robustness sensitivity six dye/buffer combinations, we then their responses metabolic activity astrobiological analog high-Arctic samples. determined that Biolog Inoculating Fluid (IF)-C AlamarBlue buffered IF-0a (aB-IF0a) combinations were optimal, as they detected from fewest cells (102 cells/mL) while maintaining efficacy over broad physiochemical range pH (0-13), temperature (-10°C 37°C), salinity perchlorate (tested up 30%), presence Mars regolith simulant (MMS-2). The μMAMA, which incorporated these dyes, active cold-adapted high Arctic sites, including samples amended with substrates targeting chemolithoautotrophic metabolisms. Given μMAMA's small size (we estimate complete planetary instrument could occupy little 3 L) potential for automation, it easily into almost any landed platform missions.